Abstract: Anaerobic methane oxidizing bacteria is a kind of microorganism that can used as the sole carbon source and energy source of methane, which has broad application prospects in the degradation and conversion of coal absorbing gas. We designed the drilling fluid injection system of raw coal, degradation analysis experiment system of coal absorbing gas by anaerobic microorganism independently. The experimental results show that: 30 ℃ is the most suitable growth temperature of the anaerobic methane oxidizing bacteria, the highest degradation rate is up to 47.73%; in the range of 0 MPa to 22 MPa of injection pressure, the degradation rate of methane increased with the increasing of injection pressure, when the injection pressure is 22 MPa, the raw coal is fractured, the degradation rate increases sharply, the average degradation rate reaches to 63.79%; methane degradation rate increases with the increasing of the volume of the injected liquid, the degradation rate of methane is about 55% when the bacterial suspension is 35 mL.