Abstract: Aiming at the gas explosion accidents in China, we proposed a new method to design training content, that is, from the perspective of statistics of accident cases. According to 40 cases of major explosion accidents from 2006 to 2015, statistics showed that there is 164 unsafe acts in total, of which 152 is caused by insufficient safety knowledge, accounting for 92.68%; the number of violations of Coal Mine Safety Regulations is 183, accounting for 91%, and violations of other regulations accunting for 9%. Six types of unsafe acts with the highest frequency of occurrence are consistent with six knowledge modules with the highest frequency of violations. And 95% of employees’ missing safety knowledge comes from these six knowledge modules. The study concludes that the design of training content for prevention of gas explosion accidents can be based on the Coal Mine Safety Regulations, and mainly covers the six modules of gas prevention, underground blasting, ventilation, underground fire zone management, electric, stoping and roof control. The method based on statistics of accident cases provides new idea for designing training content of preventing gas explosion accidents.