Abstract: In order to understand the degradation effect of low concentration methane in air flow by microorganism, aerobic methane consumption microorganism that takes methane as the sole carbon source in the process of cultivation was cultured; we designed a low concentration methane degradation simulation experiment system in the air flow independently to reach the target of regulate methane concentration in the air flow, the wind speed and concentration of bacteria. The experiments show that in the condition of keeping constant methane oxidizing bacteria liquid spraying speed, the higher the concentration of the bacteria, the more conducive to the degradation of methane; under the condition of keeping same methane concentration in the air flow, in the range of wind speed (300 mL/min to 500 mL/min), the higher the wind speed, the worse the degradation effect, and the effective degradation duration was also decreased; under the condition of same wind speed, methane concentration, in the range of methane concentration (10% to 30%) in the air flow, the higher the methane concentration, the better effect of the methane degradation.